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bulbs and scales: shake / stir the entire tuber/ bulb / scale in bleach
Add gelling agent (agar 6-8 g/L or gellan gum 3-4 g/L) to the media and keep stirring
waterborne
Measure 800 ml of distilled water in a beaker
Thiamine Hydrochloride (HCl) Size:10GM bulbs and scales: shake /Thiamine Hydrochloride (HCl) is indispensable in tissue culture, primarily serving as a vital coenzyme in metabolic processes. As Vitamin B1, thiamine HCl is crucial for the enzymatic decarboxylation of alpha keto acids and the oxidative decarboxylation of pyruvate to acetyl CoA, essential steps in the Krebs cycle. This metabolic pathway is fundamental for ATP production, providing the energy required for cell division, growth, and differentiation. In
