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thereby avoiding nonspecific amplification
Dilution Buffer 1
Ser-His142
30(1):448-467
RNase HII, Glycerol-free(2 U/μL) _ 14541ES Size:1 KU thereby avoiding nonspecific amplificationRNase HII is a ribonuclease internal enzyme obtained from the Pyrococcus abyssi and recombinantly expressed in E. Coli. It recognizes the DNA rN DNA DNA double strand, cutting at the site where ribonucleotides are incorporated into DNA. However, its activity on single stranded RNA is very low, and it shows no cutting activity on dsDNA or ssDNA. RNase HII cuts at the site of a single ribonucleotide residue from the 5' end, producing a 5' phosphate
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