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Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-polyacrylamide gel electrophoresis (SDSPAGE) with Tris-glycine-SDS running buffer
4°C for up to 1 year
Room temperature reaction setup
GScript RTase Size:50 reactions Read the full protocol inThe GScript RTase is recombinant M MLV RTase expressed in E. coli and purified to homogeneity. It has lower RNase H activity and high thermal stability. The enzyme is widely used to synthesize first strand cDNA at temperatures up to 55C with increased specificity, higher yields of cDNA, and more full length product than other reverse transcriptases. It can generate cDNA from 100 bp to 12 Kb.
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